Research Technician IV, Histopathology & Tissue Shared Resource (HTSR)), Lombardi Comprehensive Cancer Center – Georgetown University Medical Center
Georgetown Lombardi Comprehensive Cancer Center (LCCC) clinicians are dedicated to providing the best professional care, delivered with compassion, for all patients. Lombardi's internationally-renowned physicians treat virtually every type of cancer, including breast, lung, colorectal, prostate, head and neck, and cancers of the blood. Along with cutting-edge cancer care, we offer a program of innovative clinical trials and a full range of supportive care services to improve our patients' health and quality of life.
The Research Technician IV provides skilled medical technology and histology capabilities to support the scope of the pathology and histology services, and the research related functions and goals of the Histopathology Laboratory in the Lombardi Cancer Center. Daily responsibilities include Immunohistochemical tissue staining, slide scanning, image acquisition and image analysis to support biomarker discovery and validation. S/he prepares billing for completed work to ensure proper payment for services provided.
The general laboratory environment requires adhering to safety procedures including universal precautions- gowns, gloves, masks, and goggles as appropriate. Work in biosafety cabinets for some procedures is included along with work in fume hood. Use of hazardous chemicals and other biohazardous agents including infectious agents. There will also be microtomy and dissection using sharp instruments. Reporting directly to both HTSR Co-Directors, the Research Technician has duties that include but are not limited to:
Manages all technical administrative aspects of experimental design for conducting assigned projects. Performs basic and translational research. Performs tests, assays and procedures appropriate to the project(s), following specific protocols. Adjusts protocols as necessary to accomplish goals. Problem-solves. Prepares and runs assays such as immunohistochemistry (IHC) and in situ hybridization, western blots, ELISA, TUNEL assay, animal dissection, tissue fixation, sectioning, cryo-sectioning, H&E staining, immunofluorescent staining, microscopic analysis of slides.
Provides image acquisition and quantitative image analysis of slides stained by dye based stains or immunohistochemistry. Designs image analysis programs for automated quantification of stained samples.
Provides analysis of results, statistical evaluation, and interpretation of data. Reports results by maintaining and updating specific database, logs, applicable to unit/area of duties and research. Maintains accurate and complete records. Develops laboratory protocols and procedures. Provides training to laboratory personnel and users. Generates invoices for billing of services.
Manages all technical administrative aspects of experimental design for conducting assigned projects. Contributes to the authoring of publications and presentations. Performs literature searches and investigations. Keeps abreast of current literature. Prepares concept proposals and grant applications for future projects and funding under the direction of the HTSR Co-Directors.
Assumes other duties and responsibilities that are appropriate to the position and area and as directed by the HTSR Co-Directors. Inventories and requests laboratory supplies necessary to the position.
Master’s degree - PhD preferred
2 to 4 years of biocomputing and laboratory experience/skills - preferably with image analysis and direct immunohistochemistry skills and/or experience with Immunohistochemistry and image acquisition and analysis
Aptitude for working with research specimens and image analysis
Knowledge of anatomy and histology
Proficiency in Microsoft office (especially PowerPoint),filing, maintenance, database management and spreadsheet applications, and Photoshop
Self-starter with detail orientation, and strong organizational, communications (both verbal and written), and problem-solving skills
Ability to document and keep complete records
Organized, has Initiative; problem solving ability
Typing and office experience skills; image processing in Power Point and Photoshop. Microsoft Office proficient
Detail orientated; good communication skills, both verbal and written
Ability to document and keep complete records
Internal Number: JR05380
About Georgetown University Medical Center
The Histopathology & Tissue Shared Resource (HTSR) is Georgetown Lombardi's resource for accessing human tissue for translational research and provides comprehensive, high quality laboratory and interpretive pathology services. The HTSR consents for, collects and distributes fresh- and formalin-fixed paraffin embedded tissue as well as provides technical and pathological support for investigator-driven collection protocols including the novel conditionally reprogrammed cells program. The HTSR also coordinates with the company Indivumed for the collection of high-quality biospecimens including matching fresh frozen and formalin fixed tissues, serum, plasma, urine and comprehensive clinical data. HTSR Co-Director Brent Harris, MD, PhD, provides comprehensive pathology services with a speciality in neuropathology. Under the direction of HTSR Co-Director Deborah Berry, PhD, the Histopathology Laboratory provides comprehensive histology services including necropsy, tissue processing, microtomy, staining, immunohistochemistry, laser capture micro-dissection and tissue microarray construction, staining and high-throughput analysis. In addition, the HTSR provides expert technical support, consultation services and educational support and training for users.
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